The BAS Lab has an automated 3130XL Genetic analyzer which has a 16 capillary 50 cm array. It can be used for DNA sequencing, genotyping and fragment analysis. Learn more about 3130XL Genetic Analyzer.
Submission information
Review guidelines for submissions.
Complete and send the sample submission form along with the samples.
The turnaround time for sequencing results are 2-3 business days.
Sample Preparation
Sequence quality will depend largely on the quality of the template. The core will process the samples and run it on the sequencer. The core uses Big Dye terminator V3.1 reagent kit from Life Technologies to process the samples.
If a customer wants the core to purify PCR products for amplicon sequencing, that fact must be clearly mentioned to the core lab personnel. The customer has to provide minimum 15 ul of raw purified PCR product for purification and the required primer in 3.2pmol/ul concentration.
Pricing
| Service | University & State Agencies' Rates | Incubator/Tenant Rates | Commercial Rates |
| DNA sequencing | $6 | $9 | Please request a quote |
| PCR product clean up and DNA sequencing | $10 | $15 | Please request a quote |
| Plasmid prep and DNA sequencing | $12 | $18 | Please request a quote |
Data analysis
The output sequencing data files include a text file (.seq) and a chromatogram file (.ab1). Chromatogram file requires specific software to open and analyze the data. Free available online software for chromatogram files include: