Our other large Zeiss epifluorescent microscope is the Zeiss Axiophot. In addition to fluorescence microscopy, this microscope is also used for phase contrast and differential interference contrast (DIC) microscopy. Though it is an older scope, it was recently outfitted with a modern digital Zeiss AxioCam MR camera and utilizes the same Zen software as the new Axio Imager 2. Phase contrast and DIC microscopy allow for observation of unstained, live cells and structures. For fluorescent microscopy, this microscope utilizes a Zeiss XBO 75 (75W xenon arc lamp) and HBO 100 (100W mercury arc lamp).
For additional information including manuals, manufacturer links, protocols and publications, see our resources page. To see images captured by this microscope, see the gallery.
| Equipment Specifications: | |||||
| LIGHT SOURCE: | Zeiss XBO-75/HBO-100 | ||||
| CAMERA: | Zeiss AxioCam MR | ||||
| OBJECTIVES: | 100x oil, 63x oil, 40x Ph1, 10x Ph2 | ||||
| ATTACHMENT(S): | Phase Contrast and DIC capabilities | ||||
| COMPUTER: | Dell Optiplex 7010 | ||||
| SOFTWARE: | Zen 2012 | ||||
| Filter Cube Specifications: | |||||
| ID | EXCITATION | BEAM SPLITTER | EMISSION | ||
| 02 | G 365 | FT 395 | LP 420 | ||
| 05 | BP 395-440 | FT 460 | LP 470 | ||
| 09 | BP 450-490 | FT 510 | LP 515 | ||
| 15 | BP 546/12 | FT 580 | LP 590 | ||
| 38 | BP 470/40 | FT 495 | BP 525/50 | ||
Click a row for detailed information about each filter set, and a list of compatible dyes!